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KMID : 1134820000290030525
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Journal of the Korean Society of Food Science and Nutrition
2000 Volume.29 No. 3 p.525 ~ p.530
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Reduction Effect of Carcinogen - induced Mouse Epidermal and Forestomach Carcinogenesis by the Extract of Onion Wastes
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Lee Chan-Joong
Kim Hee-Dae
Jeong Eun-Ho
Suh Jun-Kyu
Park Cherl-Woo
ha Yeong-Lae
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Abstract
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Effect of the onion waste extract (OWE) on chemical carcinogen-induced mouse epidermal and forestomach carcinogenesis was investigated. Female ICR mice (6～7 weeks of age) were adapted in a temperature- and humidity-controlled house for one week. In a epidermal carcinogenesis model, animals were randomly divided into 3 treatment groups (7 mice/cage, 21 mice/treatment group). Back of the mouse was shaved 10 days before 7,12-dimethylbenz[a]anthracene (DMBA) treatment. OWE (20 mg/0.2 mL acetone) or quercetin (20 mg/0.2 mL acetone) was painted on the back of the mouse on 7th-days, 3th-days and 5-min before the initiation of carcinogenesis with DMBA (50 nmole/0.2 mL acetone). Control mice were received only 0.2 mL acetone. From one week after the initiation, each mouse received twice weekly injection of phorbol 12-myristate 13-O-acetate (TPA: 6 μg/0.2 mL acetate) until the termination of experiment (23 weeks). In forestomach carcinogenesis model, female ICR mice (6～7 weeks of age, 10 mice/cage, 20 mice/treatment group) was randomly divided into 4 treatment groups: 50 mg and 25 mg OWE/0.2 mL soybean oil, 25 mg quercetin/0.2 mL soybean oil, and 0.2 mL soybean oil only. Mouse received sample through garbage on Monday and Wednesday, and benzo[a]pyrene (BP) (2 mg/0.2 mL soybean oil). This treatment process was repeated for 4 weeks. Control mice received soybean oil only. In the case of epidermal carcinogenesis, OWE treatment (1.3 tumors/mouse) reduced the number of tumors per mouse, relative to that of the control (2.9 tumors/mouse), but similar to that of quercetin treatment (1.2/mouse). The tumor incidence (85.7%) in mouse treated with OWE was slightly reduced as compared to that (95.2%) of the control, and slightly higher than that (76.2%) of quercetin treatment group. OWE treatments (50 mg and 25 mg) reduced the number of forestomach tumors per mouse to 33.7% and 31.5% of the control, respectively. However no difference was observed from quercetin treated mouse. Forestomach tumor incidence was also reduced from 100% (control) to 88.2% (50 mg OWE) and 94.1% (25 mg OWE). The tumor incidence of 50 mg OWE was similar to that (83.3%) of 25 mg quercetin treatment. These results indicate that OWE inhibited the initiation of epidermal and gastric carcinogenesis in mice, and the effective dose was similar to quercetin.
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KEYWORD
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onion waste extract, mouse epidermal carcinogenesis, mouse forestomach carcinogenesis
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